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Introduction:
Contains the components as described by Hickok, et al (1995).
This medium was developed for the germination of fern
P713 Parker Thompson Fern Basal Salt Mixture
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 0.77g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients as described by Hickok et al. (1995) Unadjusted pH = 3.5 – 4.5 (See certificate of analysis for lot-specific value.) |
| Formula (mg/L) | |
|---|---|
| Ammonium Molybdate | 0.037 |
| Ammonium Nitrate | 125 |
| Boric Acid | 1.86 |
| Calcium Chloride, Anhydrous | 19.628 |
| Cupric Sulfate.5H2O | 0.37 |
| Na2 EDTA.2H2O | 37.3 |
| Ferrous Sulfate.7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 58.565 |
| Manganese Sulfate.H2O | 0.25 |
| Potassium Phosphate, Monobasic | 500 |
| Zinc Sulfate.7H2O | 0.52 |
Application Notes
Plant Tissue Culture Tested Plant species: Ceratopteris and other fern species This medium was developed for the germination of fern spores and may need to be supplemented with a carbohydrate source and gelling agent.
References
Hickok, LG, TR Warne, and RS Fribourg. 1995. The biology of the fern Ceratopteris and its use as a model system. Int. J. Plant Sci. 156: 332-345