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Introduction:
This medium is a modification of P723 Orchid Seed Sowing Medium, except charcoal has been eliminated, and is best suited for species that do not produce phenolics. This medium may be used for some terrestrial orchid species by preparing the medium at ½ strength and supplementing it with 50 ml of coconut water per liter.
P727 Orchid Seed Sowing Medium Contains Agar, Without Charcoal
Properties
| Form: | Powder |
| Appearance: | Off-white to Cream Powde |
| Application: | Orchid Culture |
| Solubility: | Hot Water |
| Typical Working Concentration: | 31.74 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | The pH of this medium should be adjusted prior to sterilization. |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | |
| Boric Acid | 1.65 |
| Calcium Chloride, Anhydrous | 83 |
| Cobalt Chloride.6H2O | 0.0063 |
| Cupric Sulfate.5H2O | 0.0063 |
| Na2EDTA.2H2O | 18.65 |
| Ferrous Sulfate.7H2O | 13.93 |
| Magnesium Sulfate,Anhydrous | 75.18 |
| Manganese Sulfate.H2O | 4.23 |
| Molybdic Acid, Sodium Salt.2H2O | 0.0625 |
| Potassium Iodide | 0.2075 |
| Potassium Nitrate | 475 |
| Potassium Phosphate, Monobasic | 42.5 |
| Zinc Sulfate.7H2O | 2.65 |
| Agar | 8000 |
| MES (Free Acid) | 500 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 1 |
| Peptone from Meat | 2000 |
| Pyridoxine.HCl | 1 |
| Sucrose | 20,000 |
| Thiamine.HCl | 10 |
Application Notes
Plant Tissue Culture Tested
Plant species: Phalaenopsis, Epiphytic orchids
This medium is a modification of P723 Orchid
Seed Sowing Medium, ex
cept charcoal has been
eliminated, and is best suited for s
pecies that do not produce phenolics.
This medium may be used for some terrestrial
orchid species by preparing the medium at ½
strength and supplementing it with 50
ml of coconut water per liter.
Approximately 4 g/L of agar
should also be added.
(Revised 12/2012)