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Introduction:
This medium is a modification of our original orchid replate medium (P668). It is a complete replate medium containing banana powder to promote growth & rooting, and a gelling agent (agar) for physical support. This medium should be pH adjusted prior to sterilization.
P748 Orchid Maintenance/Replate Medium Contains Banana, Charcoal, and Agar Replate Medium I
Properties
| Form: | Powder |
| Appearance: | Gray powde |
| Application: | Orchid Cultur |
| Solubility: | Partially Soluble in Wat |
| Typical Working Concentration: | 64.31 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | pH = 4.5 - 5.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 825 |
| Boric Acid | 3.1 |
| Calcium Chloride, Anhydrous | 166 |
| Cobalt Chloride.6H2O | 0.0125 |
| Cupric Sulfate.5H2O | 0.0125 |
| Na2 EDTA.2H2O | 37.3 |
| Ferrous Sulfate.7H2O | 27.85 |
| Magnesium Sulfate, Anhydrous | 90.35 |
| Manganese Sulfate.H2O | 8.45 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.125 |
| Potassium Iodide | 0.415 |
| Potassium Nitrate | 950 |
| Potassium Phosphate, Monobasic | 85 |
| Zinc Sulfate.7H2O | 5.3 |
| Activated Charcoal | 2000 |
| Agar | 7000 |
| Banana Powder | 30,000 |
| MES (Free Acid) | 1000 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 1 |
| Peptone from Meat | 2000 |
| Pyridoxine.HCl | 1 |
| Sucrose | 20,000 |
| Thiamine.HCl | 10 |
Application Notes
Plant Tissue Culture Tested
Plant species: Many epiphytic orchid species
This medium is a modification of our original or
chid replate medium (P668
). It is a complete
replate medium containing banan
a powder to promote growth
& rooting, and a gelling agent
(agar) for physical support. This medium s
hould be pH adjusted prio
r to sterilization.