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Introduction:
This medium is a modification of our Orchid Multiplication Medium (P723) by the deletion of charcoal and agar. It was originally developed for the multiplication of plantlets from Phaleonopsis flower stem nodal segments. This medium when used in combination with Orchid Maintenance/ Replate Medium (P668 or P748) provides a complete plant propagation cycle.
This medium has been used for protocorm production of Cattleya and Cymbidium species. The pH of this medium should be adjusted after adding a gelling agent and prior to sterilization.
P793 Orchid Multiplication Medium Without Charcoal and Agar
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Orchid Cultur |
| Solubility: | Water |
| Typical Working Concentration: | 25.30 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | pH = 4.75 - 5.75 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 825 |
| Boric Acid | 3.1 |
| Calcium Chloride, Anhydrous | 166 |
| Cobalt Chloride.6H2O | 0.0125 |
| Cupric Sulfate.5H2O | 0.0125 |
| Na2EDTA.2H2O | 37.3 |
| Ferrous Sulfate.7H2O | 27.85 |
| Magnesium Sulfate,Anhydrous | 90.35 |
| Manganese Sulfate.H2O | 8.45 |
| Molybdic Acid, Sodium Salt.2H2O | 0.125 |
| Potassium Iodide | 0.415 |
| Potassium Nitrate | 950 |
| Potassium Phosphate, Monobasic | 85 |
| Zinc Sulfate.7H2O | 5.3 |
| 6-Benzylaminopurine (BA) | 2 |
| MES (Free Acid) | 1000 |
| myo-Inositol | 100 |
| a-Naphthaleneacetic Acid | 0.5 |
| Nicotinic Acid (Free Acid) | 0.5 |
| Peptone from Meat | 2000 |
| Pyridoxine.HCl | 0.5 |
| Sucrose | 20,000 |
| Thiamine.HCl | 1 |
Application Notes
Plant Tissue Culture Tested
Plant species: Many epiphytic orchid species
This medium is a modification of our Orchid Mu
ltiplication Medium (P72
3) by the deletion of
charcoal and agar. It was or
iginally developed for the mult
iplication of plantlets from
Phalaenopsis flower stem nodal s
egments. This medium when us
ed in combination with Orchid
Maintenance/ Replate Medium (P668 or P748) prov
ides a complete plant propagation cycle.
This medium has been used for protocorm pr
oduction of Cattleya and Cymbidium species.
The pH of this medium should be adjusted after
adding a gelling agent and prior to sterilization. (Revised 12/2012)