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Introduction:
Rugini Olive Medium is useful for the micropropagation of some types of olive cultivars (Rugini 1984).
It contains the macronutrients, micronutrients, and vitamins as described by E. Rugini in 1984.
R7100 Rugini Olive Medium
Properties
| Form: | Powder |
| Appearance: | White to Light Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | 4.05g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by E. Rugini (1984). Plant Tissue Culture Tested Unadjusted pH (approx): 3.5 - 4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 412 |
| Boric Acid | 12.4 |
| Calcium Chloride Anhydrous | 332.16 |
| Calcium Nitrate, Anhydrous | 416.9 |
| Cobalt Chloride 6H2O | 0.025 |
| Cupric Sulfate 5H2O | 0.25 |
| Na2 EDTA 2H2O | 37.5 |
| Ferrous Sulfate 7H2O | 27.8 |
| Magnesium Sulfate Anhydrous | 732.5 |
| Manganese Sulfate H2O | 16.9 |
| Molybdic Acid, Na Salt 2H2O | 0.25 |
| Potassium Chloride | 500 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1100 |
| Potassium Phosphate, Monobasic | 340 |
| Zinc Sulfate 7H2O | 14.3 |
| D-Biotin | 0.05 |
| Folic Acid | 0.5 |
| Glycine | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid | 5 |
| Pyrodoxine HCl | 0.50 |
| Thiamine HCl | 0.50 |
Application Notes
Useful for the micropropagation of some types of olive cultivars (Rugini 1984).
References
Rugini E (1984) In vitro propagation of some olive (Olea europaea L.) cultivars with different root ability, and medium development using analytical data from developing shoots and embryos. Scientia Horticulturae 24: 123-134.