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Introduction:
Contains the macro- and micronutrients and vitamins as described by Schenk and Hildebrandt (1972). Also contains 10 g/L Sucrose.
S813 Schenk & Hildebrandt Modified Basal Medium Contains Vitamins and 10 g/L Sucrose
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 14.21g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Schenk and Hildebrandt (1972). Also contains 10 g/L Sucrose. pH = 3.75 – 4.75 |
| Formula (mg/L) | |
|---|---|
| Ammonium Phosphate, Monobasic | 300 |
| Boric Acid | 5 |
| Calcium Chloride, Anhydrous | 151 |
| Cobalt Chloride.6H2O | 0.1 |
| Cupric Sulfate.5H2O | 0.2 |
| Na2 EDTA.2H2O | 20 |
| Ferrous Sulfate.7H2O | 15 |
| Magnesium Sulfate, Anhydrous | 195.4 |
| Manganese Sulfate.H2O | 10 |
| Molybdic Acid (Sodium Salt).2H2O | 0.1 |
| Potassium Iodide | 1 |
| Potassium Nitrate | 2500 |
| Zinc Sulfate.7H2O | 1 |
| myo-Inosito | 1000 |
| Nicotinic Acid (Free Acid) | 5 |
| Pyridoxine•HCI | 0.5 |
| Sucrose | 10,000 |
| Thiamine•HCl | 5 |
Application Notes
Plant Tissue Culture Tested
References
Schenk, RU & AC Hildebrandt. 1972. Medium and Techniques for Induction and Growth of Monocotyledonous and Dicotyledonous Plant Cell Cultures. Can. J. Bot. 50: 199-204.