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Introduction:
Contains a modification of the macro- and micronutrients, glucose, and agar as described by Steele (1996).
Contains 600 mg/L Calcium Nitrate and 200 mg/L Casein. Without Ammonium Nitrate.
T842 Terrestrial (Cypripedium) Orchid Medium
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Orchid Culture |
| Solubility: | Partially Soluble in Water |
| Typical Working Concentration: | 27.44 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains a modification of the macro- and micronutrients, glucose, and agar as described by Steele (1996). Contains 600 mg/L Calcium Nitrate and 200 mg/L Casein Without Ammonium Nitrate pH = 4.75 - 5.75 |
| Formula (mg/L) | |
|---|---|
| Ammonium Citrate | 19 |
| Boric Acid | 0.5 |
| Calcium Nitrate | 600 |
| Cupric Sulfate.5H2O | 0.025 |
| Ferric Ammonium Citrate | 25 |
| Magnesium Sulfate, Anhydrous | 97.69 |
| Manganese Sulfate.H2O | 1.54 |
| Molybdic Acid, Sodium Salt.2H2O | 0.02 |
| Potassium Chloride | 100 |
| Potassium Iodide | 0.1 |
| Potassium Nitrate | 200 |
| Potassium Phosphate, Monobasic | 200 |
| Zinc Sulfate.7H2O | 0.5 |
| Agar | 6000 |
| Casein, Enzymatic Hydrolysate | 200 |
| D-Glucose | 20,000 |
Application Notes
Plant Tissue Culture Tested
Plant species: Cypripedium and other terrestrial orchid species
References
Steele, WK. 1949. In: C. Allen, Editor, North American Native Terrestrial Orchids. Propagation and Production Conf. Proc., pp 11-26. (Revised 3/2007)