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Introduction:
Contains the macro- and micronutirents as described by Coke (1996a).
W865 Westvaco WV5 Basal Medium
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 5.22g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Coke (1996). |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 700 |
| Boric Acid | 31 |
| Calcium Chloride, Anhydrous | 452.88 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.25 |
| Ferric Sodium EDTA | 36.7 |
| Magnesium Sulfate, Anhydrous | 903.79 |
| Manganese Sulfate.H2O | 15.16 |
| Molybdic Acid (Sodium Salt).2H2O | 0.25 |
| Potassium Chloride | 718.67 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1084.06 |
| Potassium Phosphate,Monobasic | 270 |
| Zinc Sulfate.7H2O | 8.6 |
| myo-Inositol | 1000 |
| Thiamine.HCl | 0.4 |
Application Notes
Plant Tissue Culture Tested Plant species: Pinus spp.
References
Coke, JE. 1996. US patent 5,534,434.