| Life Tech Providing Service |
Studies undertaken |
Service Code |
| In Vitro Models for Early Drug Discovery |
| In Vitro Cell Based Drug Discovery |
| Cardiovascular Disease |
Cardioprotective studies |
SC-ST00B |
| Hypertrophic cardiomyopathy related studies |
| Myocardial Infarction based studies |
| Atrial fibrillation (AF) (cardiac arrhythmia) based studies |
| hERG : Patch-clamp assay |
| hERG serum shift assays |
| Ion channel trafficking assay |
| Action potential duration (ADP) assays |
| Cardiac marker detection: Troponin I, II |
| Integrated human cardiomyocyte assays |
| Hit Identification |
| High content imaging- analysis of drug interaction at the sub-cellular level |
| Miscellaneous based on customers requirement subject to Feasibility |
| Immunology |
| In-Vitro Immunology Assays for Drug Screening: |
Fibroblast-like synoviocyte activation assay |
SC-ST00B |
| Chemotaxis assay: Monocytes |
| Chemotaxis assay: Neutrophils |
| Conventional Dendritic cells activation assay |
| Plasmacytoid Dendritic cells activation assay |
| Anti-inflammatory assays |
| Immunomodulatory assays |
| Clinical Immunology Assays |
Flow cytometry for PK/PD |
SC-ST00B |
| Flow cytometry for CD marker analysis of immune cells |
| ELISpot, to evaluate cellular immune response |
| Multiplex cytokine analyses |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Metabolic Disorders |
| ANTI-DIABETES |
Cell proliferation studies |
SC-ST00B |
| Glucose uptake assay |
| Alpha-amylase inhibition assay |
| Alpha-glucosidase inhibition assay |
| Insulin receptor binding studies |
| IGF-R1 receptor binding studies |
| Skeletal muscle regeneration assays using stem cells models |
| Assays monitoring the protection of human podocytes or freshly isolated glomeruli |
| Assays measuring anti-fibrotic effects across different primary cell types |
| A comprehensive set of primary islet cell assays covering all relevant aspects of pancreatic beta-cell turnover and function |
| Protection/reversal of metabolic stress-induced beta-cell de-differentiation |
| Cell-specific primary rat and human beta cell replication (plated or intact islets) |
| Cell-specific quantification of beta-cell apoptosis in plated islets |
| Insulin secretion studies with rat and human islets |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Oncology |
| Cytotoxicity & Proliferation Assays |
SRB assay |
SC-ST00B |
| ATP assay |
| LDH assay |
| Cell cycle |
| MTT assay |
| Ki67 assay, |
| Clonogenic assay |
| Apoptosis and DNA Damage Studies |
Annexin V/PI assay |
SC-ST00B |
| Ao/EtBr assay |
| p53 assay |
| Caspases assay |
| 21 assay |
| Comet assay |
| MMP - JC1 assay |
| Cyt-C assay |
| Cell cycle arrest |
| NO analysis |
| TUNEL assay |
| ROS analysis |
| Angiogenesis and Metastasis Assays |
Cell migration assays |
SC-ST00B |
| Scratch assay |
| Tube formation assay |
| Angiogenesis signalling pathways (HIF1α, TGF-b, VEGF, SMAD, p38, Pi3) |
| Cancer Signalling Pathway Marker Studies |
C-Jun test |
SC-ST00B |
| cAMP assay |
| HIF-1 alpha assay |
| STAT 1,2,3, & 6 assay |
| Phospho-mTOR assay |
| Phosphor-Akt (p-Akt) assay |
| P53 expression |
| Phosphor-ERK assay |
| Ca+2 assay |
| NF-kappa B/p65 |
| Phosphor-CREB assay |
| EGFR assay |
| Akt assay |
| P38 activity assay |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Neural Disorder |
| Monitor Cell-Cell Interactions Between Vascular and Brain Cells |
Real-time drug permeability across the BBB. |
SC-ST00B |
| Neurotoxicity on the cells of the BBB. |
| Evaluate drugs that have a protective effect on the BBB |
| Investigate the effects of tumor cells on the BBB |
| Evaluate inflammatory effects on the BBB |
| Biomarker Analysis |
| Visualize and quantify the migration of immune cells |
| Genomic, proteomic, and metabolic analysis of normal and dysfunctional BBB. |
| Neurodegenerative Diseases: Alzheimer's and Parkinson's Disease |
Tau levels/ clearance assays |
SC-ST00B |
| Toxic Aβ1-42 peptide exposure |
| LDH release measurement |
| NeuN cell viability assay |
| Alpha-synuclein aggregation quantification |
| Neuronal mitophagy assays (Tom20 Loss Quantification) |
| Cytoprotective assays |
| Neuro-Inflammation |
Lipopolysaccharide (LPS)-induced inflammation |
SC-ST00B |
| Nitric oxide production |
| Microglia activation |
| Macrophage activation |
| Phagocytosis Assays |
| Human microglia assays: cytokine release & calcium imaging |
| Neuronal and microglial inflammation assays |
| Microglial activation & phagocytosis studies |
| Neuro-Glia co-cultures studies |
| Cytokine release assays |
| LPS-induced inflammation |
| Neurite outgrowth assays |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Renal Disorders |
| Renal Disorders |
Cytotoxicity Assays |
SC-ST00B |
| Life Technologies includes measurement of cell health markers like GSH, PLD, mito mass, MMP, and ATP levels. |
| Mitochondrial Stress Assessment |
| Biomarker (KIM-1 and NGAL) Detection |
| High Content Screening- Using standard cell model and disease-related cell lines. |
| High Content Imaging- Analysis of drug interaction at a sub-cellular level |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Respiratory disease |
| Respiratory disease |
Human Primary cell-based assays- Using 2D and 3D disease-relevant models |
SC-ST00B |
| High Content Screening- Using standard cell model and disease-related cell lines. |
| Inhalation toxicity test |
| Anti-viral studies |
| In-vitro 3D respiratory models- for drug testing, inhalation toxicity, viral infections and inflammation studies |
| 3D mucociliary tissue model - human-derived nasal/bronchial epithelial cells |
| The 3D model derived from basal cells, goblet cells and ciliated cells |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Liver Diseases |
| Non-Alcoholic Fatty Liver Disease (NAFLD) and Non-Alcoholic Steatohepatitis (NASH) |
Life Technologies assists in establishing NASH conditions by modulating glucose, fructose, free fatty acids, and lipopolysaccharide (LPS) levels. |
SC-ST00B |
| Cytotoxicity assays |
| Cytokines analysis: IL-6, TNF-α, MIP-1α (CCL3), MCP-1, IP-10 (CXCL10), IL-8 (CXCL8) |
| Expression of profibrotic markers |
| Apoptosis marker studies |
| Oxidative stress marker studies |
| Mitochondrial dysfunction studies |
| Cellular lipids and phospholipids estimation by LipidTox staining |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Colon Diseases |
| Inflammatory bowel disease (IBD)/CROHN’S |
Cytotoxicity and proliferation assays |
SC-ST00B |
| Pro and anti-inflammatory marker studies |
| Gut microbiome analysis by NGS |
| Host–microbiome interactions and pathways |
| Measurement of ALP, ZO-1, MMP2, MMP9, MUC2, MUC5AC, and ADAMTS1 |
| Stem Cells Research for Degenerative Diseases |
Isolation and Characterization |
SC-ST00B/SC-PT00B |
| Isolation and maintenance of adult tissues and umbilical cord blood/tissue-derived stem cells. |
| Characterization of MSCs/AMSCs/HSCs/NSCs by flow cytometry |
| 2D and 3D model-based Differentiation Studies |
| Neuronal Differentiation Studies |
| Osteogenic Differentiation Studies |
| Adipogenic Differentiation Studies |
| Cardiomyocytes Differentiation Studies |
| Dendritic and other immune cell generation from HSCs/MSCs |
| Trans-differentiation studies |
| Signalling pathways governing stem cells: Notch, Wnt, Hedgehog etc |
| Wound Healing |
Proliferation assays |
SC-ST00B |
| Cell migration assays: Scratch and cell inserts based |
| Angiogenesis assays: Tube formation assay, CAM assay |
| Anti-inflammatory assays: Pro and Anti-inflammatory cytokines studies |
| Endocrine Disruption Screening |
Estrogen Receptor (ER) Transactivation |
SC-ST00B |
| Androgen Receptor (AR) Transactivation |
| Androgen Receptor (AR) Modulation |
| Estrogen Receptor-alpha (ER-α) Binding |
| Estrogen Receptor beta (ER-β) Binding |
| Androgen Receptor (AR) Binding |
| Steroidogenesis |
| Aromatase Assay |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Infectious Diseases |
| Viral Indicators |
COVID-19, SARS, H1N1, MERS and Ebola, Influenza, Hepatitis B and C |
SC-ST00B |
| Bacterial Indicators |
Expertise in multi drug-resistant infections as clA, cUTl, HAP/VAP, ABSSI, joint infections, osteomyelitis. |
SC-ST00B |
| In-Vitro Antimicrobial studies |
MIC assays (minimum inhibitory concentration)
MBC assays (minimum bactericidal concentration)
Time-kill assays
In Vitro pharmacokinetic and pharmacodynamics assays
Resistance testing
Cell-based assays (internalization and killing and biofilm assays)
Immune modulation assays (ELISA, Luminex, and FACS)
Metagenomics |
SC-ST00B |
| In Vitro Anti-Viral studies |
COVID-19 protease inhibition assay |
SC-ST00B |
| TCID50 assay |
| EC50 /CC50 assay |
| Plaque assays |
| HAI assays (hemagglutination inhibition) |
| Metagenomics |
| Gastrointestinal Disorders |
Ascites |
SC-ST00B |
| Biliary Duct Stenosis |
| Colorectal Conditions |
| Constipation |
| Crohn's Disease |
| Liver Cirrhosis |
| Exocrine pancreatic insufficiency in cystic fibrosis |
| GERD |
| Intestinal transit modulation |
| Inflammatory Bowel Disease |
| Irritable Bowel Disease |
| Gastroenteritis Infections |
| Pancreatic cancer |
| Ulcerative Colitis |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Additional Discovery Services |
| Microbiome Research |
Whole Genome Sequencing (WGS) |
SC-ST00B |
| Exome sequencing |
| RNA sequencing |
| Pre- implantation screening |
| Prenatal and postnatal screening |
| Hybridoma or antibody sequencing |
| Agriculture and livestock genotyping |
| Metagenomics |
| Microbiome analysis |
| Microbial identification: 16s, 18s and STR sequencing |
| SNP analysis |
SC-ST00B |
| Nanoparticles in Drug Delivery |
Particle size analysis |
| Particle count |
| SEM |
| TEM |
| UV and XRD |
| Zeta potential |
| FTIR |
| Cytotoxicity and other biological applications |
| Molecular Biology-Based Services |
Cloning, sequencing, and expression service |
SC-ST00B |
| gDNA extraction, Reverse transcriptase PCR and Real-time PCR |
| RNA extraction, cDNA synthesis, Real-time PCR and Report |
| Protein purification service from recombinant Bacteria (His tag) and wild fungi strains |
| Labelling services |
| DNA fingerprinting service/RAPD |
| Mycoplasma detection by PCR |
| Endotoxin detection by colorimetry/fluorimetry |
| Epigenetics services include methylation, miRNA, and DNA-protein interaction analysis. |
| Gene Synthesis |
Standards Gene synthesis |
OS-TS00C |
| ProLongGene |
| DNA Fragment |
| SsDNA |
| PCR Cloning & Subcloning |
| Mutagenesis |
| Plasmid Preparation |
| Oligo Synthesis |
Custom DNA Oligos |
OS-TS00C |
| Custom RNA Oligos |
| qPCR Probes |
| NGS Oligos |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Bioanalytical Laboratory Services |
| Phytochemistry |
Plant extractions, isolation of secondary metabolites and bioactive compounds. |
SC-ST00B/SC-IK00B |
| Bioanalytical Services |
GC-MS, LC-MSMS, SEM, TEM, HPLC, HPTLC, NMR, X-RD. |
| Biomarker Testing |
Flow cytometry, Immunocytochemistry - Confocal microscopy, ELISA, WB. |
| Immunology Testing |
Immunohistochemical and immunocytochemical assays, ELISA-based assays |
| Next-generation Sequencing |
GWAS studies, SNP detection, RNA seq, Exome Sequencing, CNV Analysis |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Industries Based Discovery Services |
| Biotech/Biopharma |
| Small Molecules Discovery Services |
|
SC-ST00B/SC-IK00B |
| Large Molecules Discovery Services |
|
SC-ST00B/SC-IK00B |
| Recombinant DNA Technology |
Cloning and Vector Generation |
SC-ST00B/SC-IK00B |
| Ligation dependent and independent cloning |
| Recombination based high throughput cloning |
| De novo cloning (Synthetic gene fragments, PCR and cDNA based) |
| Customized vector construction based on client requirement |
| Plasmid DNA Preparation |
Research grade plasmid at small scale suitable for cloning, mutagenesis, microbial transformation and protein production. |
SC-ST00BSC-IK00B |
| High quality industrial grade plasmid suitable for protein, antibody production, stable cell line generation in mammalian cells, bacmid generation for protein expression in insect cells, viral packaging, in vitro mRNA synthesis, vaccine, gene therapy studies and animal immunization. |
| Process development for customized plasmid propagation and purification. |
| Mutagenesis |
Customized mutagenesis specifications, including the creation of mutant libraries and site-directed and random mutagenesis. |
SC-ST00B/SC-IK00B |
| Point mutations, insertions, deletions, or substitutions of gene fragments. |
| Alanine scanning, combinatorial mutagenesis, site saturation mutagenesis, and gene shuffle. |
| Antibody Gene Sequencing and Reformatting |
RACE (Rapid Amplification of cDNA Ends) using template switching adapter ligation. |
SC-ST00B/SC-IK00B |
| Degenerate oligos. |
| De novo antibody sequencing and analysis using LC-MS/MS. |
| Reformatting antibody gene sequences into various other production formats like bispecifics, mAb, scFv, Fab, F (ab)'2, VHH/Nanobody. |
| Antibody gene sequencing for Mouse, Rat, Rabbit and other species. |
| Strain Engineering |
BSL-1 and BSL-2 microbes |
SC-ST00B/SC-IK00B |
| Gene knockout and knockin techniques based on homologous recombination. |
| Characterization of engineered strains using microbiological methods, RT-PCR and advanced analytics. |
| CRISPR-Cas9 based mutagenesis. |
| Gene Expression Analysis |
Quantitative real time PCR. |
SC-ST00B/SC-IK00B |
| ELISA in various formats. |
| Flow Cytometry. |
| Western blotting. |
| Mass Spectrometry. |
| Residual Protein, DNA and Mycoplasma Detection |
Quantitative Real time PCR |
SC-ST00B/SC-IK00B |
| ELISA |
| SPR (Biacore) |
| Mycoplasma test |
| Protein Sciences: Protein Expression |
Designing, scouting and screening studies for challenging proteins. |
SC-ST00B/SC-IK00B |
| Protein expression studies. |
| With low running expenses, scale up using the spinner flask, shaker flask, HYPER flask, and WAVE bioreactor platforms to handle volumes up to 50 L scale. |
| Mammalian Expression Systems |
Mammalian systems used for expression are CHO, ExpiCHO, HEK, ExpiHEK, etc. |
SC-ST00B/SC-IK00B |
| Protein production in transient expression mode or using stable clones or pools. |
| Insect Cell Expression Systems |
|
SC-ST00B/SC-IK00B |
| Yeast Expression Systems |
|
SC-ST00B/SC-IK00B |
| Bacterial Expression Systems |
Soluble, periplasmic and inclusion bodies in E. coli |
SC-ST00B/SC-IK00B |
| Multi-protein expression by co-transfection or duet plasmid technology |
| Refolding condition screening and method optimization |
| Protein Purification |
Purification process optimization |
SC-ST00B/SC-IK00B |
| Automated FPLC systems and batch binding protein purification |
| IMAC, IEC, mixed-mode, HIC, SEC, and affinity for micrograms to gram-scale quantities are chromatographic purification techniques. |
| Antibody Purification |
Purification of monoclonal antibodies from various sources, including human and non-human primates, mice, rats, pigs, gerbils, and more. |
SC-ST00B/SC-IK00B |
| Generation from full-length mAbs and purification of various antibody fragments (Fab, Fab2, F(ab)'2, VHH, scfv, etc.) |
| Bi-specific and KIH antibodies |
| Diabodies |
| Single domain antibody |
| Protein purification from hybridoma cultures |
| Microbial Purification Capabilities |
Soluble protein |
SC-ST00B/SC-IK00B |
| Periplasmic protein |
| Intracellular protein |
| Refolding from inclusion bodies |
| Purification of Major Histocompatibility (MHC) complexes |
| Membrane protein production - GPCRs, nanodisc technology, etc. |
| Antibody drug conjugate screening, development and purification |
| High throughput protein screening and purification |
| Protein cleavage and purification |
| Protein Analytics and Characterization |
Quantitative analysis: Protein estimation using Absorbance at 280nm,Bradford and BCA assays |
SC-ST00B/SC-IK00B |
| Qualitative analysis: SDS-PAGE (Reducing/Non-reducing) and DOT/Western blotanalysis |
| Purity: SE-HPLC, IEX-HPLC and RP-HPLC |
| Protein stability analysis (Real-time and Accelerated Stability Testing) |
| LC-MS analysis |
| Capillary electrophoresis (CE). |
| Peptide Mass Fingerprinting (PMF) |
| Biological activity and binding studies using enzymatic assays, ELISA, Biacore, etc. |
| Endotoxin analysis |
| Host cell protein (HCP) and Host cell DNA (HCD) analysis |
| Protein Stability Analysis |
Real-time and accelerated stability testing. |
SC-ST00B/SC-IK00B |
| Long-term stability studies. |
| Purity: SE-HPLC, IEX-HPLC and RP-HPLC |
| Protein Conjugation and Labelling |
Protein labelling/conjugation (HRP, biotin, Ruthenium, fluorescent labels, etc.) |
SC-ST00B/SC-IK00B |
| Conjugation of resin with specific protein/peptide |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Biologics Testing Solutions |
| Contamination & Impurity Testing |
|
|
| Bioactivity & Potency Testing |
Method development, transfer, and optimization |
|
| Phase-appropriate method validation |
|
| Stability storage and testing |
SC-ST00B/SC-EX00B |
| Accelerated stress studies |
|
| Comparability testing between innovator and biosimilar biological products |
|
| Lot & Final Drug Product Release Testing |
Identity: |
SC-ST00B/SC-EX00B |
| SDS-PAGE with western blot or CE-SDS |
| Isoform analysis (IEF, C-IEF) |
| Potency |
| Cell-based potency assays |
| In vivo potency |
| Protein characterization |
| Binding assays |
| Purity and Impurities: |
| Residual DNA/host cell protein |
| Size-exclusion HPLC of proteins |
| Cation-exchange HPLC of proteins |
| Reverse-phase (RP) HPLC of proteins |
| CE-SDS, C-IEF |
| Physiochemical Properties: |
| pH determination |
| Osmolality determination |
| Presentation attributes |
| Visual Assessment |
| Determination of volume in syringes and vials |
| Determination of excipient levelsn |
| Microbiology |
| Sterility testing |
| Microbiology assays |
| LAL/monocyte activation test, in vivo pyrogenicity |
| General safety/abnormal toxicity |
| Protein Characterization |
Ultra-high-pressure liquid chromatography (UHPLC) |
SC-ST00B/ SC-EX00B |
| Mass Spectrometry (MS) |
| Cell Line Characterization |
We find out the origin and history of the cell line by checking its cellular morphology and growth characteristics |
SC-ST00B/ SC-EX00B |
| Cellular identity |
| Purity of the cell lines (i.e., absence of contaminating cells, microbial contaminations, and contaminations by adventitious viruses) |
| Tumorigenicity and oncogenicity |
| Stability |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Coveted Services |
| Hybridoma Cell Line Development |
|
SC-AG00J |
| Antibody Production |
1) Monoclonal Antibody Production in Ascites (Mice)
2) Monoclonal Antibody Production (Hybridoma Cell Culture) - Proprietary Method to Increase Yield
3) Recombinant Antibody Production in CHO Cells from Sequence
4) Polyclonal Antibody Production (Rabbit or Goat) |
Development of Recombinant Producing
Protein Cell Lines in Yeast or E.Coli |
|
| GMP Manufacturing of Recombinant Proteins |
1) Protein A Purification of Antibodies
2) Protein G Purification of Antibodies
3) Immunoaffinity Column Development and Purification of Antibodies
4) Affinity Chromatography Purification HPLC, for Proteins |
1) HRP Conjugation
2) ALP Conjugation
3) Biotin Labeling |
|
| Epitope Binning: Develop Matched Pair of Antibodies for Use in Assays |
|
| Total Development and Validation of ELISA Kit. |
|
| Total Development and Validation of Rapid Lateral Flow Strip. |
|
| Exosome related Services |
Exosome related Services:-
1.Exosome isolation from cell culture medium
Exosome isolation from serum
2.Dynamic Light scattering for exosome or nanoparticle size and zeta potential detection) (DLS analysis)
3. Nanoparticle Tracking analysis to detect number of exosome particle & size in 1 ml sample)
4. Transmission electron microscopy to detect structure and size of exosomes or nanoparticle
5. HPLC assay to quantify amount of drug loading in exosomes/ nanoparticles
6. Drug or natural compound packaging in exosomes (exogeneous packaging) |
| MTT Assay Service |
Testing the toxicity of new anti-cancer molecules on primary tumor cells, CTCs (MTT Assay) |
| Testing the toxicity of new anti-cancer molecules on Cancer stem cell (3D culture) derived from cell lines (MTT Assay) |
| MTT assay (cell cytotoxicity assay) in a single cell line with 1 test compound) |
| Cancer stem cell characterization using CSC specific markers from a single cell line |
| Confocal microscopy to detect cellular apoptosis using AnnexinV |
| Generation of mature dendritic cells from monocytes |
| PBMC from Human blood 15 million cells (Healthy donor) |
| Flow cytometry analysis to detect Ki67 and AnnexinV |
| ELISA assay |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Cosmetic Industry |
| Verify cell lines against the database to identify the non-listed ones |
|
SC-ST00B/ SC-DI00B |
| Perform STR profile testing |
|
| Compare STR profile with donor or database |
|
| Determine the percentage that matches the given sample |
|
| Skin studies |
Photo aging, oxidant-induced damage |
SC-ST00B/ SC-DI00B |
| Hyperpigmentation |
| Wrinkle formation |
| Skin sagging |
| Loss of color |
| Surface roughness |
| Dry skin |
| Skin sensitization |
| Skin glow |
| human skin biopsies (Treated vs Untreated) |
Metagenomics by Next generation Sequencing (NGS) |
SC-DI00B |
| Serum lipidsincluding triglycerides |
| Vitamins – LC-MSMS |
| Calcium |
| Blood urea nitrogen |
| Creatinine, Elastin and Collagen levels by Q-PCR |
| In vitro Model for Skin Penetration Study |
In vitro Skin Penetration Study by using EpiDerm SystemTM |
SC-DI00B |
| In vitro Skin Penetration Study by using Strat-M Transdermal Diffusion Membrane System |
| In vitro Skin Penetration Study by using Keratinocytes cell line based assays |
| In vitro Skin Model to test Skin Health |
Matek/ EpiDerm or other 3D culture systems - EpiDerm is a proven in vitro model system for chemical, pharmaceutical and skin care product testing. |
SC-DI00B |
| Cultured at the air-liquid interface (ALI), EpiDerm allows for the evaluation of topically applied compounds, chemicals, cosmetic/personal care product ingredients and final formulations |
| In vitro tests on 3D skin culture |
Vitamin C - RT PCR, ELISA |
SC-DI00B |
| Vitamin E - RT PCR, ELISA |
| Glutathione |
| Keratin protein |
| Vitamin C transport proteins The Sodium-Dependent Vitamin C Transporters (SVCT1 and SVCT2) - RT PCR |
| Collagen and elastin synthesis |
| Barrier Lipids/Hydration - LC/MS |
| Apoptosis – DNA fragmentation assay / Comet assay |
| Upregulation of type І procollagen production by active compounds |
|
SC-DI00B |
| Modulation of UVB-induced MMPs by the active compounds |
|
SC-DI00B |
| Skin elasticity, hydration molecular markers |
|
SC-DI00B |
| Hair Studies |
Follicular general health study in Human hair by Anagen Vs Catagen molecular markers quantitative expression (in vivo) |
SC-DI00B |
| Hair follicular Bulge stem cell stimulation/activation study by quantified expression of markers (in vitro) |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Medical Device |
| Medical Device |
Bacterial Endotoxin testing for all injectable and implantable devices |
SC-ST00B/SC-PT00B |
| Biocompatibility testing |
| In-vitro hemolysis assay for hemocompatibility test |
| In-vitro cytotoxicity screening with 2D and 3D model |
| In-vitro genotoxicity biocompatibility testing |
| Bacterial reverse mutation test (AMES) |
| In vitro mouse Lymphoma assay TK |
| In vitro micronucleus test |
| In vitro chromosome aberration test |
| In-vitro cardiotoxicity testing |
| Quantification of Total Organic Carbon as part of cleaning evaluation of reusable medical devices. |
| Quantification of bacterial bioburden as part of cleaning evaluation using Inoculated simulated test soil. |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Agrochemical & Biocide |
| Agrochemical & Biocide |
In-vitro cytotoxicity using 2D and 3D tissue models |
SC-ST00B |
| Skin corrosion, skin sensitization, skin irritation models |
| Phototoxicity and ocular damage study models |
| Other tissues- lung, mouth and gut. |
| In-vitro Endocrine disruption studies |
| In-vitro dermal absorption studies |
| Tolerance, Safety & Metabolism studies through ADME and PK profiling |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Nutraceuticals & Dietary Supplements |
| Nutraceuticals & Dietary Supplements |
Microbial Identification:- |
SC-ST00B |
| Detecting and identifying microbial strains |
| Metagenomics |
| Bacterial, fungal, and viral genome sequencing |
| 16s, 18s, RNA Sequencing |
| Bacterial endotoxin testing services |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| In Vitro ADME/DMPK |
| In Vitro ADME/DMPK |
Physico-Chemical Assays: |
SC-ST00B |
| In Vitro Metabolism |
| Protein Binding |
| Permeability and Transporters |
| Cytochrome P450 Induction |
| Metabolite Identification and Biotransformation |
| In Vivo Pharmacokinetics |
| Pre-Formulation Services |
| Bioanalysis Quantitation |
| PK/PD Modelling and Simulations |
| Large Molecule Analysis |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Toxicology and Safety Assessment |
| Hepatotoxicity & Drug-induced Liver Injury (DILI) |
| In Vitro Toxicity Panel at Life Technologies |
Using HepG2, HUH7, Hep3B cell lines and human iPSC HepRG spheroid cultures |
SC-ST00B |
| Drug-induced phospholipidosis and steatosis |
| Lysosomal trapping (lysosomotropic) |
| Cholestasis |
| Mitochondrial permeability transition |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Multiparametric Toxicity |
| Multiparametric Toxicity Assessment Services |
increased likelihood of clinical hepatotoxicity prediction (sensitivity of 70% and specificity of 100%) |
SC-ST00B |
| Extended panel for testing the metabolic competency of rat primary hepatocytes and HepG2 replicating cells for the presence of toxicologically important endpoints. |
| To explore and detect early and late-stage toxicities, different time points are used. |
| Including data on Cmax that is actual or forecasted and can be used to correct exposure-related consequences |
| To evaluate SAR sets and identify candidates with the lowest risk for continued development, common toxicity signatures can be used. |
| In Vitro Toxicity Solutions |
IC50 determination |
SC-ST00B |
| Cell loss/death using fluorescent imaging |
| Nuclear size and morphology using fluorescent imaging |
| Cell membrane permeability using fluorescent imaging |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Genotoxicity |
| Mitochondrial Toxicity |
Functional mitochondrial toxicity (Seahorse) |
SC-ST00B |
| Mitochondrial respiratory complex assay (using permeabilized cells) |
| Mitochondrial biogenesis |
| Mitochondrial Glu/Gal assay |
| HCS mitochondrial toxicity |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Cardiotoxicity |
| In Vitro Assays |
hERG safety screening using Invitrogen’s Predictor™ hERG fluorescence polarization assay kit |
SC-ST00B |
| 3D microtissue-based cardiotoxicity assay using human iPSC – CM (cardiomyocytes) spheroid cultures |
| The Comprehensive in vitro Proarrhythmia Assay (CiPA) Core Panel (5 Concentrations) |
| Human iPSC-derived cardiomyocytes FLIPR Penta assays |
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Miscellaneous based on customers requirement subject to Feasibility |
|
| Neurotoxicity |
| Nephrotoxicity |
| Dermal toxicity |
| Dermal toxicity |
In vitro irritation using 3D reconstructed human epidermis models (OECD 439) |
SC-ST00B |
| In vitro skin corrosion using 3D reconstructed human epidermis models (OECD 431) |
| Phototoxicity assay using BALB/c-3T3 cells |
| Skin sensitization assay using KeratinoSens cell line (OECD 442D) |
| Skin sensitization by directing peptide reactivity assay (OECD 442C) |
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Miscellaneous based on customers requirement subject to Feasibility |
|
| Ocular toxicity |
| Ocular toxicity |
Electroretinography (ISCEV, custom protocols, and VEP) |
SC-ST00B |
| SD-OCT enables anterior and posterior imaging and retinal layer segmentation. |
| Pachymetry (corneal thickness) |
| Digital fundus photography/video |
| CSLO provides 3D images using infrared and tomography modes. |
| Fundus autofluorescence |
| Fluorescein angiography (FA) |
| Indocyanine green angiography (ICG) |
| Vitrectomy |
| Aqueous/vitreous humor collections and biomarker quantification |
| Slit-lamp bio microscopy |
| Indirect and direct ophthalmoscopy |
| Gonioscopy |
| Streak retinoscopy |
| Draize test |
| Ocular reflexes |
| In Vitro Assays |
Conducting OECD 491 assay with SIRC cell line for short-term exposure. |
SC-ST00B |
| EpiOcular™ eye irritation assay using 3D reconstructed human corneal epithelium tissue model (OECD 492) |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
| Embryotoxicity |
| Embryotoxicity |
Developmental and reproductive pathology |
SC-ST00B |
| Specialized neurobehavioral testing |
| Functional assessments cover growth, pulmonary, neurobehavioral, reproductive, and immunological development. |
| Toxicokinetic evaluations, including induction studies |
| Juvenile toxicity testing in rodents and nonrodent species |
| Screening Information Data Sets (SIDS) |
| Hormone assays |
| Male reproductive assessments in rodents and nonrodents |
| Behavioral tests encompass pre/postnatal and multigenerational studies, assessing activity, learning, and memory. |
| Range of developmental immunology tests that can be added to pre-and postnatal and multigenerational studies |
| Endocrine disruptor screenings (in vivo and in vitro) |
| Skeletal measurements include bone size, geometry, density using micro-CT, pQCT, X-ray, and DXA in pre/postnatal studies. |
| Sperm/oestrous cycle analyses |
| Neuro-histomorphometry |
| Genomics |
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Miscellaneous based on customers requirement subject to Feasibility |
|
| Plant and Agriculture Services |
GMO Protein Testing in Plants:
Cry1Ac, Cry2Ab, CP4 EPSPS, VIP3A |
Qualitative or Quantitative Testing of GMO Proteins in Plant Samples |
SC-AG00J |
| DNA Fingerprinting |
|
| Zygosity in Cotton |
Testing for Zygosity in Breeding Lines; for MON 531 MON 15985 |
| GMS Testing in Cotton |
Testing for Genetic Male Sterility Traits/Markers in Germplasm for Breeding. |
| Bioefficacy Testing |
|
| TY Genes in Tomato |
Testing a Panel of Resistance Traits for Tomato including TY markers for Tomato Leaf Curl Virus, Tobacco Mosaic Virus, Bacterial Wilt and Phytopthora. |
| Double Haploid Line Development (DH) |
| Genomic DNA extraction from plant |
SC-EX00B |
| mRNA isolation from leaf and seed |
| Sample preparation for ELISA (plant samples like tomato, potato) |
| |
Miscellaneous based on customers requirement subject to Feasibility |
|
|
Cell Line Engineering Services |
| Cell surface marker validation service |
SC-ST00B |
| Cell immortalization services |
SC-ST00B |
| Epigenetic induction of cell growth service |
SC-ST00B |
| Custom immortalized cell line development service |
SC-ST00B/SC-AG00J |
| Custom host cell line development |
SC-ST00B |
| Miscellaneous based on customers requirement subject to Feasibility |
|
| Clinical Research Services |
| Investigator and Trial Site Facilitation Services |
SC-PT00B |
| Feasibility Service |
SC-PT00B |
| Site Management Service |
SC-PT00B |
| Monitoring Service |
SC-PT00B |
| Quality Assurance Service |
SC-PT00B |
| Regulatory Service |
SC-PT00B |
| Medical Writing |
SC-PT00B |
| Insourcing Service |
SC-PT00B |
| Post Marketing Surveillance |
SC-PT00B |
| Statistical Service |
SC-PT00B |
| Non Industry Sponsored Clinical Trial Solutions |
SC-PT00B |
| Miscellaneous based on customers requirement subject to Feasibility |
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